Effects of L-carnitine on erectile function and structure in elderly male rats

LIU Yuanhua; DAI Haitao; XIONG Ying; LIU Changmao; HUANG Hui; ZHANG Zhong; ZHENG Jiang; CHENG Shaoping; GUO Linjie

doi:10.13201/j.issn.1001-1420.2017.11.014

Article navigation > J Clin Urol > 2017 > 32(11): 881-884

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LIU Yuanhua, DAI Haitao, XIONG Ying, et al. Effects of L-carnitine on erectile function and structure in elderly male rats[J]. J Clin Urol, 2017, 32(11): 881-884. doi: 10.13201/j.issn.1001-1420.2017.11.014

Citation:

Effects of L-carnitine on erectile function and structure in elderly male rats

Department of Urology, First People's Hospital of Jingzhou, Jingzhou, Hubei, 434000, China

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Corresponding author: ZHENG Jiang, E-mail: zhengjiang980712@163.com

Received Date: 09 February 2017

Abstract

Abstract

Objective: To explore the effect of L-carnitine on erectile function and structure in elderly male rats.Method: Three-month-old male rats and 16-month-old male rats were used to study.The weight and blood pressure of the 16-month-old male rats were measured after L-carnitine feeding for two months.The number of erections and yawns of rats in 30 min were observed after subcutaneous injection of apomorphine.TGF-beta 1 mRNA expression level was detected by RT-PCR.The expression level of TGF-beta 1 was detected in Western blot.F-actin fluorescence staining and confocal microscopy were used to observe the ratio of smooth muscle and collagen in the penis tissue.Result: There was no statistically significant difference in weight and blood pressure between the elderly group and the elderly L-carnitine group (P>0.05).The number of erections and yawns were significantly lower in the older group than those in the control group, and the difference was statistically significant (P<0.01).The number of erections and yawns in the elderly L-carnitine group were significantly higher than those in the elderly group, and the difference was statistically significant (P<0.01).The relative expression level of TGFbeta 1 mRNA in elderly group was significantly higher than that in the control group, and the difference was statistically significant (P<0.01).The relative expression level of TGF-beta 1 mRNA in elderly L-carnitine group was significantly lower than that in the older group, and the difference was statistically significant (P<0.01).The expression rate of TGF-beta 1 was significantly higher in the elderly group than that in the control group, and the difference was statistically significant (P<0.01).The expression rate of TGF-beta 1 protein in elderly Lcarnitine group was significantly lower than that in the elderly group, and the difference was statistically significant (P<0.01).The ratio of smooth muscle and collagen was significantly lower in the elderly group than that in the control group, and the difference was statistically significant (P<0.01).The ratio of smooth muscle and collagen in the elderly L-carnitine group was significantly higher than that in the elderly group, and the difference was statistically significant (P<0.01).Conclusion: L-carnitine can improve the erectile function in rats by inhibiting TGF-beta 1 expression.
- L-carnitine /
- rats /
- aging /
- erectile function

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References

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